Scientists Lift the Lid on The Secret Life of Manganese
A new biosensor engineered by Penn State researchers offers scientists the first dynamic glimpses of the elusive – and vital – manganese ion. The researchers engineered the sensor from a natural protein called lanmodulin, which binds rare earth elements with high selectivity and was discovered five years ago by some of this study’s researchers. Their findings are published in the Proceedings of the National Academy of Sciences.
The researchers genetically reprogrammed the protein to favour manganese over other common transition metals like iron and copper, unlike most transition metal-binding molecules. The sensor could have broad applications in biotechnology to advance understanding of photosynthesis, host-pathogen interactions and neurobiology.
Like iron, copper and zinc, manganese is an essential metal for plants and animals. Its function is to activate enzymes. In humans, manganese is linked to neural development. Accumulation of excess manganese in the brain induces Parkinsonian-like motor disease, whereas reduced manganese levels have been observed in association with Huntington’s disease, the researchers explained.
“We believe that this is the first sensor that is selective enough for manganese for detailed studies of this metal in biological systems,” said Jennifer Park, a graduate student at Penn State and lead author on the paper. “We’ve used it – and seen the dynamics of how manganese comes and goes in a living system, which hasn’t been possible before.”
She explained that the team was able to monitor the behaviour of manganese within bacteria and are now working to engineer even tighter binding sensors to potentially study how the metal works in mammalian systems.
Scientific understanding of manganese has lagged behind that of other essential metals, in part because of a lack of techniques to visualise its concentration, localisation and movement within cells. The new sensor opens the door for all kinds of new research, explained Joseph Cotruvo, associate professor of chemistry at Penn State and senior author on the paper.
“There are so many potential applications for this sensor,” said Cotruvo. “Personally, I am particularly interested in seeing how manganese interacts with pathogens.”
He explained that the body works hard to restrict the iron that most bacterial pathogens need for survival, and so those pathogens instead turn to manganese.
“We know there is this tug-of-war for vital metals between the immune system and these invading pathogens, but we haven’t been able to fully understand these dynamics, because we couldn’t see them in real time,” he said, adding that with new capabilities to visualise the process, researchers have tools to potentially develop new drug targets for a range of infections for which resistance has emerged to common antibiotics, like staph (MRSA).
Designing proteins to bind to particular metals is an intrinsically difficult problem, Cotruvo explained, because there are so many similarities between the transition metals present in cells. As a result, there has been a lack of chemical biology tools with which to study manganese physiology in live cells.
“The question for us was, can we engineer a protein to only bind to one thing, a manganese ion, even in the presence of a huge excess of other very similar-looking things, like calcium, magnesium, iron, and zinc ions?” Cotruvo said. “What we had to do was create a binding site arranged in just the right way, so that this protein bond was more stable in manganese than any other metal.”
Having successfully demonstrated lanmodulin is capable of such a task, the team is now planning to use it as a scaffold from which to evolve other types of biological tools for sensing and recovering many different metal ions that have biological and technological importance.
“If you can figure out ways of discriminating between very similar metals, that’s really powerful,” said Cotruvo. “If we can take lanmodulin and turn it into a manganese-binding protein, then what else can we do?”
Source: Penn State
