p53 is one of the most important proteins in cancer biology. Often referred to as a “guardian of the genome”, p53 activates in response to cellular stressors such as DNA damage. Its activation induces processes such as cell death that prevent cancer development. Because of this, p53 mutations are extremely common in cancers, including hepatocellular carcinoma.
However, a study published in Cancer Research, revealed that the constant activation of p53 in liver cells of patients suffering from chronic liver disease (CLD) could actually promote the development of liver cancer.
CLD can be brought on by different factors including viruses, alcohol use, and fat accumulation, all of which can induce p53 activation. Previous studies have shown that p53 is in a constant state of activation in the liver cells of CLD patients. Yet, it is not clear what role this plays in CLD pathophysiology.
“Clinical data clearly show that p53 is activated in the hepatocytes of individuals with CLD,” says Yuki Makino, lead author of the study at Osaka University. “Because p53 is such a vital part of how the human body prevents tumor formation, its role in CLD became even more intriguing.”
To create a mouse model with p53 accumulation in hepatocytes, researchers deleted Mdm2, the protein responsible for regulating p53 expression by targeting it for degradation. These mice developed liver inflammation with higher amounts of hepatocyte apoptosis and senescence-associated secretory phenotype (SASP), a phenomenon where cells produce signals within the microenvironment that can cause nearby cells to become cancerous. In fact, mice with p53 accumulation did have increased liver tumour development.
“We also observed an expanded population of hepatic progenitor cells (HPCs), which have stem cell-like characteristics,” explained senior author Tetsuo Takehara. “When the HPCs were isolated, grown in culture, and then injected under the skin of lab mice, these animals developed tumors. This suggested that HPCs played a key part in the liver tumor formation seen in the animals with p53 accumulation.”
Interestingly, acceleration of liver tumour development and the other observed phenotypes did not occur when p53 was deleted in addition to Mdm2 in the hepatocytes. These results demonstrated the significance of constant p53 activity in the tumorigenesis.
“We then compared samples from 182 CLD patients with 23 healthy liver samples,” said Dr Makino. “The CLD liver biopsy samples showed activated p53 was positively correlated with apoptosis levels, SASP, HPC-associated gene expression, and later cancer development.”
The authors concluded that constitutively activated p53 in hepatocytes of CLD patients can create a microenvironment that is supportive of tumour formation from HPCs. Their work proposes a novel and paradoxical mechanism of liver tumorigenesis because p53 is one of the most well-known tumor suppressor genes. These data could highlight p53 as a potential cancer-prevention treatment target for CLD patients.
Source: Osaka University
